The advancement in quantification of public health risks associated with bioaerosols emissions from a growing number of waste management technologies such as green waste composting is constrained by methodological challenges. The real-time characterization of bioaerosols emissions can significantly improve the estimation and modelling of their public health impact. This investigation aims to characterize bioaerosols emissions in real-time at a green waste compositing facility. Four measurements were conducted by using a novel ultraviolet light-induced fluorescence sensor, the Spectral Intensity Bioaerosol Sensor (SIBS). On average the highest number and mass concentration of the fluorescent particles were 6.02 #/cm3 and 141.05 µg/m3, respectively. The number and mass fraction of fluorescent particles ranged from 0.15 – 0.35 and 0.10 – 0.68. The count median diameter of total and fluorescent particles increased from 0.65 µm and 0.62 µm during no activity to 0.96 µm and 1.17 µm during activity (turning). The emissions of total and fluorescent particles vary temporally and the number and mass concentration of fluorescent particles during turning can increase to 5.71 #/cm3 and 46.17 µg/m3 at ~ 70 m downwind of the source. The magnitude and wavelength dependent spectral modes of emission intensity were distinctly different during activity (turning) and no activity periods. The dominant peaks aligned with coenzymes, structural biopolymers, secondary metabolites and pigments. Our findings illustrate the complex nature and magnitude of bioaerosols emissions from green waste composting and demonstrate how real-time characterization can be used to advance quantification of public health risks associated with bioaerosols emissions from environmental sources.