Impact of Spray Drying on Superoxide Dismutase Activity in Composite Systems with Optimal Aerodynamic Performance for Dry Powder Inhalers

Diana A. Fernandes1,2, Raquel Barros1, Cláudia Moura1, Eunice Costa1 & Maria L. Corvo2
Poster

The aim of this study was to investigate the effect of drying process variables on the aerodynamic performance and enzymatic activity of a spray-dried model enzyme, Cu,Zn-Superoxide Dismutase (Cu,Zn-SOD). Cu,Zn-SOD is often implicated in a broad spectrum of oxidative stress related diseases, from Cystic Fibrosis to Rheumatoid Arthritis, catalyzing the breakdown of superoxide radicals and so providing the first line of defense against oxygen toxicity. A 23 full factorial experiment was conducted at a constant Drying Gas Flowrate using a trehalose:leucine based composite system previously optimized for dry powder inhalers [1]. Cu,Zn-SOD:trehalose:leucine spray dried powders were successfully generated displaying Fine Particle Fraction values (FPFs) of ≈60% and Enzymatic Activity Retention (EAR) values ranging 50-80%. Aerodynamic performance of these SD powders was mainly affected by Ratom, while enzymatic activity was mainly influenced by Ratom and Ffeed.
Introduction

Join today to view and download the full abstract/presentation