Bacterial transport in aerosols is a key step in the transmission of diseases such as Tuberculosis[1] (TB) and pneumonia.[2] These diseases create a large global health burden, causing the deaths of millions of people every year.[3] Studying the way bacteria behave within aerosol droplets, and the factors influencing their survival, will be important in developing countermeasures to limit the spread of these diseases. We have developed a system for creating and suspending bacterial aerosols in a controlled and reproducible manner, allowing for detailed measurements to be made on the impact of various parameters on bacterial survival.