Efficient and Rapid Recovery of bioaerosol DNA From the Air

Robert. Ferguson, Corinne Whitby, Alex Dumbrell, and Ian Colbeck

Annual Aerosol Science Conference 2016

Podium

We optimised methods for recovery of genetic material from bacterial bioaerosols with a number of samplers. Polycarbonate filters and PBS were the best performing filter and impingement liquids respectively. The sampling time (2hrs) was too short to recover DNA with the Andersen sampler at urban and agricultural sights but data from the compost sight provided proof of concept for the sampling strategy. Syringe filters did not perform as well as our lab based protocol but show promise as a rapid and cheap method for DNA recovery on sight, in subsequent work we will trial this with portable qPCR for on sight pathogen detection.

Join today to view and download the full abstract/presentation

Become a member

The Aerosol Society

Members

The Aerosol Society recognises the importance of our diverse community and believe that to produce the best quality science we must encourage a proactive and inclusive approach to equality. This can be achieved by developing and retaining a diverse range of talented people to achieve their full potential free from discrimination. We acknowledge our role in engaging with the wider aerosol science community to develop a fair and friendly environment by promoting equality, diversity and inclusion for all, recognising that we come from varying backgrounds and experiences.