The Micro device is being developed for the delivery of aerosolized drugs. We tested 3 Micro devices at 5°C and 30°C to evaluate the impact of temperature on the performance of the Micro device. The devices were placed in a 5°C refrigerator for over 2 hours before being weighed, filled with 300 μL of 5 mg/2.5 mL salbutamol sulphate solution equilibrated to 5°C, and reweighed. The Micro device was connected to a Malvern Spraytec laser diffraction system via a custom connector and was operated until completion of treatment. Aerosolization time was recorded and the device was reweighed before being placed in a 30°C incubator for at least 2 hours, and the test was then repeated. This protocol was repeated for each of the 3 devices. The delivered dose and volume median diameter particle size results were consistent across the 3 Micro devices tested and with the 2 different temperatures. Output rate was lower at 5°C; however, as the delivered dose was unchanged, this only resulted in an increased aerosol delivery time (from 29 seconds at 30°C, to 39 seconds at 5°C). Overall, the results indicate that the Micro device performance was consistent in the key parameters of aerosol output at temperatures of 5°C and 30°C, which are outside the typical operating range of the Micro device.