Aerosol measurements from e-cigarette devices in an in vitro Air-Liquid Interface (ALI) cell exposure system

Aerosol measurements from e-cigarette devices in an in vitro Air-Liquid Interface (ALI) cell exposure system

Matthew D Wright1, Nathan Goldsmith1, Jelizaveta Zuikova1,2, Tim Marczylo1

1Centre for Radiation, Chemical & Environmental Hazards (CRCE), Public Health England, Chilton, Didcot, OX11 0RQ, UK

2Centre for Biomedical Education, St George’s University of London, Cranmer Terrace, London, SW17 0RE, UK

 

Summary

E-cigarette use is increasing rapidly, but health impact assessments are controversial and based on limited knowledge. In addition, the physicochemical properties of e-cigarette emissions are not well characterised, and the method of measurement and exposure may impact on measurement of the size distribution, chemical composition, dose estimate and hence toxicological response induced by e-cigarette aerosol. We present an experimental setup to examine the effect of e-cigarette aerosol exposure to human bronchial epithelial cells in vitro in an Air-Liquid Interface (ALI) system, designed to replicate aerosol thermodynamic behaviour along the path from device-to-lung in ‘real-world’ use. Initial characterisation based on particle number concentration and retrieved nicotine concentration is presented, and subsequent work on both aerosol characterisation and exposure studies is outlined.

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